Bacteria and virus samples can be directly used as templates for PCR after being processed with reagent in the kit, without extracting microbial genomic DNA, thus effectively avoiding possible cross contamination due to sample purification. The kit is suitable for rapid detection of clinical bacteria and foodborne bacteria.
· Convenient—Buffer formulated for primer annealing at 60°C, helping simplify PCR optimization
· Fast—No DNA purification, no cross contamination
· Efficient— High inhibitor tolerance; GC enhancer included for amplification of GC-rich sequence
AJdirect Microbial (Yeast/Bacterial) PCR Kit
AJdirect Microbial (Yeast/Bacterial) PCR Kit
AJ-4300-50
AJ-4300-200
50 T/package
200 T/package
A. Blood sample containing bacteria and virus
· Take 50μL blood sample in a 1.5mL tube, add 100μL Lysis Buffer AB and 10 μL Inhibitor Remove, vortex.
· Incubate at 95 ℃ water bath for 10 minutes.
· Centrifuge at 9,000 rpm for 1 minute, and the supernatant can be used as the DNA template for PCR.
B. Swab samples
· Adding 200μL Lysis Buffer AB into a 1.5mL centrifuge tube, immerse the swab head directly into the Lysis Buffer, and repeatedly squeeze it against the tube wall for several times and then discard the swab. Add 10 μL Inhibitor Remove, vortex.
· Incubate at 95 ℃ water bath for 10 minutes.
· Centrifuge at 9,000 rpm for 1 minute, and the supernatant can be used as the DNA template for PCR.
C. Foodborne samples
· Foods were put into a sterile container and minced, a small piece of food sample was mixed with 10mL of cell cluture medium and incubated at 37℃ for 6- 12 hours.
· Transfer 50 μL cell culture to a 1.5mL centrifuge tube and add 100 μL Lysis Buffer AB and 10 μL Inhibitor Remove, vortex.
· Incubate at 95 ℃ water bath for 10 minutes.Centrifuge at 9,000 rpm for 1 minute, and the supernatant can be used as the DNA template for PCR.